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Ramu, A.
- Formulation and Evaluation of Lovastatin Solid Dispersions with Pregelatinised Starch as Newer Superdisintegrant
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Affiliations
1 Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522 510, Andhra Pradesh, IN
2 Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur - 522 019, Andhra Pradesh, IN
1 Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522 510, Andhra Pradesh, IN
2 Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur - 522 019, Andhra Pradesh, IN
Source
Journal of Pharmaceutical Research, Vol 11, No 2 (2012), Pagination: 38-43Abstract
Solid dispersions of lovastatin were formulated using pregelatinised starch (PGS) as super disintegrant and were further compressed into tablets by using various diluents such as lactose, dicalcium phosphate (DCP) and microcrystalline cellulose (MCC) to enhance the bioavailability. The solid dispersions were prepared by physical mixing, solvent evaporation and kneading methods. The solid dispersions were found to release the drug faster than the pure drug in dissolution media. The rapid release of poorly soluble lovastatin from solid dispersions was influenced by the proportion of polymer and the method employed for its preparation. Among the three methods employed solvent evaporation and kneading methods were found to be suitable for improving the dissolution rate of lovastatin. The release data was fitted to various kinetic models. The release was found to follow first order kinetics. Some of the dispersions prepared by the solvent evaporation method and kneading method were formulated into tablets with various diluents. The tablet preparations containing different diluents were found to release the drug in the order of DCP>MCC>Lactose. The dissolution rate of tablet formulations prepared with lovastatin solid dispersions (FK1, FS4) were found to release the drug at a faster rate than that of tablets prepared with plain drug.Keywords
Lovastatin, Pregelatinised Starch, Solid Dispersions.- Neuroprotective Effects of Isolated Fraction from Sapindus laurifolia Extract in Hippocampal Neuronal HT22 Cells
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Authors
Affiliations
1 Department of Pharmaceutical Sciences, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Guntur, Andhra Pradesh, IN
1 Department of Pharmaceutical Sciences, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Guntur, Andhra Pradesh, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 1&2 (2020), Pagination: 79-85Abstract
Glutamate is a major endogenous excitatory neurotransmitter in the brain and contributes to the development of neurodegenerative diseases by excessive activation. The purpose of the present study was to determine the neuroprotective effect of Sapindus laurifolia (MESL) Fraction A against glutamate-induced oxidative stress and to assess the underlying mechanism. MESL Fraction A was subjected to a neuroprotective effect assay in HT22 mouse hippocampal cells. The mechanism underlying the neuroprotective effect of MESL Fraction A was evaluated by assaying Reactive Oxygen Species (ROS) levels, intracellular Ca2+ levels, mitochondrial membrane potential and glutathione level and antioxidant enzyme activity in HT22 cells. MESL Fraction A significantly decreased glutamate-induced death of HT22 cells (88.23 ± 1.65% relative neuroprotection). MESL Fraction A reduced the intracellular ROS and Ca2+ levels and increased the glutathione level and glutathione reductase and glutathione peroxide activities. Moreover, MESL Fraction A attenuated the mitochondrial membrane potential in HT22 cells. These results suggested that MESL Fraction A exerts a neuroprotective effect against oxidative stress HT22 cells, which was mediated by its antioxidant activity.Keywords
Antioxidant, Glutamate, Hippocampal Cells, Neuroprotective, Oxidative Stress.References
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